Is it possible to use GPIO53 ~ GPIO56 SPI mode in 32bit mode?
--You cannot use hardware SPI pins in 32-bit mode. However, you can override the GPIO-GPIO and use them as bit-banged SPI pins.
You can go through the example: C:\Program Files (x86)\Cypress\EZ-USB FX3 SDK\1.3\firmware\serialif_examples\cyfxusbspigpiomode
The following example implements the same functionality as you want to implement.
And is it possible to use GPIO50 ~ GPIO52 GPIO mode in 32bit mode?
--You can use these as GPIO's but you will have to firstly override them using CyU3PDeviceGpioOverride() API and then configure them as simple or complex.
You can go through the implementation in SDK example from the path: C:\Program Files (x86)\Cypress\EZ-USB FX3 SDK\1.3\firmware\serialif_examples\cyfxgpioapp