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Here are the steps:
1.Open the GPIF project provided with AN65974.
2.Select 16 bit as Data Bus width in the Interface settings. (GPIF Designer Tool)
3.Save it and build the project by clicking Build > Build Project to generate the cyfxgpif2config.h file.
4.Set CY_FX_SLFIFO_GPIF_16_32BIT_CONF_SELECT to ‘0’ in the cyfxslfifosync.h file.
5.Build the project. This step generates the image file for the 16-bit Slavefifo application.