1. Which Dev Kit are you using CYUSB3KIT-001 or CYUSB3KIT-003?
2. What is the firmware did you load? Did you flash the image file to SPI Flash? or System RAM or I2c EEPROM?
3. Which PMODE setting did you configure, PMODE[2:0]= ?
4. Can you program USB Bulk Loop Auto (located at C:\Program Files (x86)\Cypress\EZ-USB FX3 SDK\1.3\firmware\dma_examples\cyfxbulklpauto) to System RAM and let me know?